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Affiliate of the American Osteopathic Association What is Prolotherapy. Prolotherapy is the stimulation of the formation of fibrous connective tissue of the body in a specific location by the specific application of a regenerative modality. Advanced Treatment for Pain Arising From Whiplash Injuries and Neck Pain Knee Conditions Ankle Foot Pain Plantar Fasciitis Shoulder Pain Rotator Cuff TearsTendinopathy Tennis and Golfers Elbow Wrist Pain Herniated Disc Pain Pain from Osteoarthritis Mid and Low Back Pain Varicose Vein Therapies We now have an updated informational brochure available for purchase. How Does Prolotherapy Work.